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primo schaer  (Addgene inc)


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    Structured Review

    Addgene inc primo schaer
    Primo Schaer, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primo+schaer/pSUMO2+(Plasmid+%2352259)/bio_rxiv__2025__09__29__679180-149-23-31
    Average 93 stars, based on 4 article reviews
    primo schaer - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Implications of critical nodes-dependent unidirectional cross-talk between Plasmodium and Human SUMO pathway proteins in Plasmodium infection
    Article Snippet: .. The Pf -SUMO wild type clone (pSUMO1-1S) for in bacto SUMOylation was derivatized from pSUMO1, was a gift from Primo Schaer (Department of Biomedicine, University of Basel; Addgene plasmid #52258). .. All Pf -SUMO and Pf -Ubc9 mutants were generated using Q5 Site-Directed Mutagenesis kit (NEB #E0554S) in Pf -SUMO wild type backbone.

    Article Title: Coronin1C SUMOylation modulates filopodia formation, neuritogenesis, and neuronal differentiation
    Article Snippet: .. The COR1C lysine mutants were generated using the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s protocol. pSUMO1/2 was a gift from Primo Schaer (Department of Biomedicine, University of Basel; Addgene plasmid # 52258, #52259) . .. The following antibodies were used for Western blotting: anti-GST (1:5000, Hi-media), anti-His (1:2000, #BB-AB0010S, Biobharti), anti-SUMO1 (76-86, DSHB) anti-GFP (1:5000; #sc-9996 Santa Cruz Biotechnology), anti-GAPDH (1:3000; #10011 Abgenex), anti-COR1C (Proteintech-14749-1-AP), Goat anti-mouse AlexaFluor Plus 800 (1:40,000, #A32730, Thermo Fisher Scientific), Goat anti-rabbit AlexaFluor Plus 680 (1:40,000, #A32734 Thermo Fisher Scientific).

    Article Title: Implications of critical node-dependent unidirectional cross-talk of Plasmodium SUMO pathway proteins.
    Article Snippet: .. The Pf-SUMO wild-type clone (pSUMO1-1S) for in bacto SUMOylation was derivatized from pSUMO1, and was a gift from Primo Schaer (Department of Biomedicine, University of Basel; Addgene plasmid #52258). .. All Pf-SUMO and Pf-Ubc9 mutants were generated using Q5 Site-Directed Mutagenesis kit (NEB #E0554S) in Pf-SUMO wild-type backbone.

    Article Title: PARP1 promotes replication-independent DNA double-strand break formation after acute DNA-methylation damage
    Article Snippet: Nuclease assay reactions were quenched with equal volume of TBE– urea sample buffer and denatured at 95°C for 5 min. After a quick spin, samples from nuclease assays were examined on 6% TBE–urea PAGE gel and imaged with a Bio-Rad ChemiDoc MP Imaging System. .. Recombinant His-tagged human APE1 protein (His-APE1) was expressed and purified from E.coli using plasmid pET28HIS-hAPE1, a gift from Primo Schaer (Addgene plasmid #70757; http://n2t.net/addgene:70757 ; RRID: Addgene 70757) . ..

    Generated:

    Article Title: Coronin1C SUMOylation modulates filopodia formation, neuritogenesis, and neuronal differentiation
    Article Snippet: .. The COR1C lysine mutants were generated using the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s protocol. pSUMO1/2 was a gift from Primo Schaer (Department of Biomedicine, University of Basel; Addgene plasmid # 52258, #52259) . .. The following antibodies were used for Western blotting: anti-GST (1:5000, Hi-media), anti-His (1:2000, #BB-AB0010S, Biobharti), anti-SUMO1 (76-86, DSHB) anti-GFP (1:5000; #sc-9996 Santa Cruz Biotechnology), anti-GAPDH (1:3000; #10011 Abgenex), anti-COR1C (Proteintech-14749-1-AP), Goat anti-mouse AlexaFluor Plus 800 (1:40,000, #A32730, Thermo Fisher Scientific), Goat anti-rabbit AlexaFluor Plus 680 (1:40,000, #A32734 Thermo Fisher Scientific).

    Mutagenesis:

    Article Title: Coronin1C SUMOylation modulates filopodia formation, neuritogenesis, and neuronal differentiation
    Article Snippet: .. The COR1C lysine mutants were generated using the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s protocol. pSUMO1/2 was a gift from Primo Schaer (Department of Biomedicine, University of Basel; Addgene plasmid # 52258, #52259) . .. The following antibodies were used for Western blotting: anti-GST (1:5000, Hi-media), anti-His (1:2000, #BB-AB0010S, Biobharti), anti-SUMO1 (76-86, DSHB) anti-GFP (1:5000; #sc-9996 Santa Cruz Biotechnology), anti-GAPDH (1:3000; #10011 Abgenex), anti-COR1C (Proteintech-14749-1-AP), Goat anti-mouse AlexaFluor Plus 800 (1:40,000, #A32730, Thermo Fisher Scientific), Goat anti-rabbit AlexaFluor Plus 680 (1:40,000, #A32734 Thermo Fisher Scientific).

    Recombinant:

    Article Title: PARP1 promotes replication-independent DNA double-strand break formation after acute DNA-methylation damage
    Article Snippet: Nuclease assay reactions were quenched with equal volume of TBE– urea sample buffer and denatured at 95°C for 5 min. After a quick spin, samples from nuclease assays were examined on 6% TBE–urea PAGE gel and imaged with a Bio-Rad ChemiDoc MP Imaging System. .. Recombinant His-tagged human APE1 protein (His-APE1) was expressed and purified from E.coli using plasmid pET28HIS-hAPE1, a gift from Primo Schaer (Addgene plasmid #70757; http://n2t.net/addgene:70757 ; RRID: Addgene 70757) . ..

    Purification:

    Article Title: PARP1 promotes replication-independent DNA double-strand break formation after acute DNA-methylation damage
    Article Snippet: Nuclease assay reactions were quenched with equal volume of TBE– urea sample buffer and denatured at 95°C for 5 min. After a quick spin, samples from nuclease assays were examined on 6% TBE–urea PAGE gel and imaged with a Bio-Rad ChemiDoc MP Imaging System. .. Recombinant His-tagged human APE1 protein (His-APE1) was expressed and purified from E.coli using plasmid pET28HIS-hAPE1, a gift from Primo Schaer (Addgene plasmid #70757; http://n2t.net/addgene:70757 ; RRID: Addgene 70757) . ..



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    93
    Addgene inc primo schaer
    Primo Schaer, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primo+schaer/pSUMO2+(Plasmid+%2352259)/bio_rxiv__2025__09__29__679180-149-23-31
    Average 93 stars, based on 1 article reviews
    primo schaer - by Bioz Stars, 2026-09
    93/100 stars
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